Western Blot:Article Title: Herpes Simplex Virus Type 2 Glycoprotein H Interacts with Integrin v 3 To Facilitate Viral Entry and Calcium Signaling in Human Genital Tract Epithelial Cells
Article Snippet: .. Primary antibodies and dilutions for Western blots, confocal microscopy, and coimmunoprecipitation were as follows: mouse monoclonal antibody (MAb) anti-VP16, 1:500 (catalog no. sc-7545; Santa Cruz Biotechnology [sc], Santa Cruz, CA); anti-VP5 MAb, 1:200 (sc-56989); anti-gB MAb, 1:500 (sc-69799), goat anti-gB, 1:200 (sc-22090); anti-gD MAb, 1:1,000 (sc-56988); anti-gC MAb, 1:200 (sc-69801); anti-gH-gL MAb, 1:200 (CH31, gift from R. Eisenberg and G. Cohen, University of Pennsylvania); anti- -actin MAb, 1:5,000 (A-5441; Sigma-Aldrich, St. Louis, MO); anti-phospho-Akt (ser 473) MAb, 1:500 (4051; Cell Signaling Technology Inc., Danvers, MA); anti-histone H1 MAb, 1:250 (sc-8030), rabbit anti-total Akt123, 1:1,000 (sc-8312); rabbit anti-pY397 focal adhesion kinase (FAK), 1:1,000 (44- 625G; Invitrogen), anti-total FAK MAb, 1:1,000 (610087; BD Bioscience), rabbit anti-goat immunoglobulin G (IgG), 1:500 (HAF017; R&D, Minneapolis, MN); goat polyclonal anti-integrin 8, 1:200 (sc-6638); goat polyclonal anti-integrin 6, 1:200 (sc-6633); rabbit polyclonal anti-integrin v, 1:500 (sc6617-R); mouse anti-integrin v, 1:200 (sc-9969); mouse anti-integrin v 3, 1:200 (sc7312); mouse anti-integrin 3, 1:50 (ab7167, Abcam, Cambridge, MA); rabbit anti-gH-gL, 1:500 (R137; gift from R Eisenberg and G. Cohen, University of Pennsylvania); rabbit polyclonal anti-PVRL1 (nectin-1), 1:100 (ab66985); and rabbit polyclonal anti-integrin 3, 1:500 (sc-14009). .. The secondary antibodies for Western blots were horseradish peroxidase-conjugated goat anti-mouse (170-5047, BioRad, Hercules, CA), goat anti-rabbit (170-5046, Bio-Rad), or donkey anti-goat (sc-2020).
Article Title: Herpes Simplex Virus Type 2 Glycoprotein H Interacts with Integrin αvβ3 To Facilitate Viral Entry and Calcium Signaling in Human Genital Tract Epithelial Cells
Article Snippet: .. Primary antibodies and dilutions for Western blots, confocal microscopy, and coimmunoprecipitation were as follows: mouse monoclonal antibody (MAb) anti-VP16, 1:500 (catalog no. sc-7545; Santa Cruz Biotechnology [sc], Santa Cruz, CA); anti-VP5 MAb, 1:200 (sc-56989); anti-gB MAb, 1:500 (sc-69799), goat anti-gB, 1:200 (sc-22090); anti-gD MAb, 1:1,000 (sc-56988); anti-gC MAb, 1:200 (sc-69801); anti-gH-gL MAb, 1:200 (CH31, gift from R. Eisenberg and G. Cohen, University of Pennsylvania); anti-β-actin MAb, 1:5,000 (A-5441; Sigma-Aldrich, St. Louis, MO); anti-phospho-Akt (ser 473) MAb, 1:500 (4051; Cell Signaling Technology Inc., Danvers, MA); anti-histone H1 MAb, 1:250 (sc-8030), rabbit anti-total Akt123, 1:1,000 (sc-8312); rabbit anti-pY 397 focal adhesion kinase (FAK), 1:1,000 (44-625G; Invitrogen), anti-total FAK MAb, 1:1,000 (610087; BD Bioscience), rabbit anti-goat immunoglobulin G (IgG), 1:500 (HAF017; R&D, Minneapolis, MN); goat polyclonal anti-integrin β8, 1:200 (sc-6638); goat polyclonal anti-integrin β6, 1:200 (sc-6633); rabbit polyclonal anti-integrin αv, 1:500 (sc6617-R); mouse anti-integrin αv, 1:200 (sc-9969); mouse anti-integrin αvβ3, 1:200 (sc7312); mouse anti-integrin β3, 1:50 (ab7167, Abcam, Cambridge, MA); rabbit anti-gH-gL, 1:500 (R137; gift from R Eisenberg and G. Cohen, University of Pennsylvania); rabbit polyclonal anti-PVRL1 (nectin-1), 1:100 (ab66985); and rabbit polyclonal anti-integrin β3, 1:500 (sc-14009). .. The secondary antibodies for Western blots were horseradish peroxidase-conjugated goat anti-mouse (170-5047, Bio-Rad, Hercules, CA), goat anti-rabbit (170-5046, Bio-Rad), or donkey anti-goat (sc-2020).
Confocal Microscopy:Article Title: Herpes Simplex Virus Type 2 Glycoprotein H Interacts with Integrin v 3 To Facilitate Viral Entry and Calcium Signaling in Human Genital Tract Epithelial Cells
Article Snippet: .. Primary antibodies and dilutions for Western blots, confocal microscopy, and coimmunoprecipitation were as follows: mouse monoclonal antibody (MAb) anti-VP16, 1:500 (catalog no. sc-7545; Santa Cruz Biotechnology [sc], Santa Cruz, CA); anti-VP5 MAb, 1:200 (sc-56989); anti-gB MAb, 1:500 (sc-69799), goat anti-gB, 1:200 (sc-22090); anti-gD MAb, 1:1,000 (sc-56988); anti-gC MAb, 1:200 (sc-69801); anti-gH-gL MAb, 1:200 (CH31, gift from R. Eisenberg and G. Cohen, University of Pennsylvania); anti- -actin MAb, 1:5,000 (A-5441; Sigma-Aldrich, St. Louis, MO); anti-phospho-Akt (ser 473) MAb, 1:500 (4051; Cell Signaling Technology Inc., Danvers, MA); anti-histone H1 MAb, 1:250 (sc-8030), rabbit anti-total Akt123, 1:1,000 (sc-8312); rabbit anti-pY397 focal adhesion kinase (FAK), 1:1,000 (44- 625G; Invitrogen), anti-total FAK MAb, 1:1,000 (610087; BD Bioscience), rabbit anti-goat immunoglobulin G (IgG), 1:500 (HAF017; R&D, Minneapolis, MN); goat polyclonal anti-integrin 8, 1:200 (sc-6638); goat polyclonal anti-integrin 6, 1:200 (sc-6633); rabbit polyclonal anti-integrin v, 1:500 (sc6617-R); mouse anti-integrin v, 1:200 (sc-9969); mouse anti-integrin v 3, 1:200 (sc7312); mouse anti-integrin 3, 1:50 (ab7167, Abcam, Cambridge, MA); rabbit anti-gH-gL, 1:500 (R137; gift from R Eisenberg and G. Cohen, University of Pennsylvania); rabbit polyclonal anti-PVRL1 (nectin-1), 1:100 (ab66985); and rabbit polyclonal anti-integrin 3, 1:500 (sc-14009). .. The secondary antibodies for Western blots were horseradish peroxidase-conjugated goat anti-mouse (170-5047, BioRad, Hercules, CA), goat anti-rabbit (170-5046, Bio-Rad), or donkey anti-goat (sc-2020).
Article Title: Herpes Simplex Virus Type 2 Glycoprotein H Interacts with Integrin αvβ3 To Facilitate Viral Entry and Calcium Signaling in Human Genital Tract Epithelial Cells
Article Snippet: .. Primary antibodies and dilutions for Western blots, confocal microscopy, and coimmunoprecipitation were as follows: mouse monoclonal antibody (MAb) anti-VP16, 1:500 (catalog no. sc-7545; Santa Cruz Biotechnology [sc], Santa Cruz, CA); anti-VP5 MAb, 1:200 (sc-56989); anti-gB MAb, 1:500 (sc-69799), goat anti-gB, 1:200 (sc-22090); anti-gD MAb, 1:1,000 (sc-56988); anti-gC MAb, 1:200 (sc-69801); anti-gH-gL MAb, 1:200 (CH31, gift from R. Eisenberg and G. Cohen, University of Pennsylvania); anti-β-actin MAb, 1:5,000 (A-5441; Sigma-Aldrich, St. Louis, MO); anti-phospho-Akt (ser 473) MAb, 1:500 (4051; Cell Signaling Technology Inc., Danvers, MA); anti-histone H1 MAb, 1:250 (sc-8030), rabbit anti-total Akt123, 1:1,000 (sc-8312); rabbit anti-pY 397 focal adhesion kinase (FAK), 1:1,000 (44-625G; Invitrogen), anti-total FAK MAb, 1:1,000 (610087; BD Bioscience), rabbit anti-goat immunoglobulin G (IgG), 1:500 (HAF017; R&D, Minneapolis, MN); goat polyclonal anti-integrin β8, 1:200 (sc-6638); goat polyclonal anti-integrin β6, 1:200 (sc-6633); rabbit polyclonal anti-integrin αv, 1:500 (sc6617-R); mouse anti-integrin αv, 1:200 (sc-9969); mouse anti-integrin αvβ3, 1:200 (sc7312); mouse anti-integrin β3, 1:50 (ab7167, Abcam, Cambridge, MA); rabbit anti-gH-gL, 1:500 (R137; gift from R Eisenberg and G. Cohen, University of Pennsylvania); rabbit polyclonal anti-PVRL1 (nectin-1), 1:100 (ab66985); and rabbit polyclonal anti-integrin β3, 1:500 (sc-14009). .. The secondary antibodies for Western blots were horseradish peroxidase-conjugated goat anti-mouse (170-5047, Bio-Rad, Hercules, CA), goat anti-rabbit (170-5046, Bio-Rad), or donkey anti-goat (sc-2020).
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